product information
Background

Soybean (Glycine max) is a plant from legume family with a broad use in food industry.
Immunogen

Glycine max flour protein extract
Host Chicken
Clonality Polyclonal
Clone
Purity Affinity purified IgY
Format Liquid at 1,2 µg/µl in PBS pH 7.2 + 0.1 % sodium azide
Quantity 100 µg
Reconstitution
Storage

store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Western blot (WB)
Related products

antibodies to food proteins

Plant protein extraction buffer

Secondary antibodies
Additional information

Antibodies were purified on immobilized soybean proteins.
application information
Recommended dilution

0.1-1 µl/ml (WB), 2- 5 µl/ml (capture ELISA)
Expected | apparent MW
Confirmed reactivity soybean proteins
Predicted reactivity

soybean proteins
Not reactive in

no confirmed exceptions from predicted reactivity known in the moment
Additional information

to be added when available
Selected references

to be added when available
application example
\"western1 µg (2) and 5 µg (3) of soy protein extract were separated on4-12%NuPage(Invitrogen)LDS-PAGEand blotted 1h toPVDF. Filters were blocked 1h with 2% low-fatmilk powderin TBS-T (0.1% TWEEN 20) and probed withanti-soy protein antibody(AS09 569, 1:10 000, 0.5µl/5ml, 1h) and secondary anti-chicken (1:25 000, 1 h) antibody (HRP conjugated, secondary antibodyAS09 602) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with TMA-6 (Lumigen) using a Fuji LAS-3000 CCD (300s, standard sensitivity). Exposure time was 2 min.